Scandinavian Journal of Laboratory Animal Sciences <p>Published by the Scandinavian Society for Laboratory Animal Science, Sweden</p> <p>Online ISSN <strong>2002-0112</strong></p> en-US (Hanna-Marja Voipio) (Timo Päivärinta) Tue, 09 Mar 2021 00:00:00 +0200 OJS 60 Buprenorphine, but not lidocaine, effectively attenuates post-operative thermal hypersensitivity in an incisional model in neonatal rats (Rattus norvegicus) <p>There is limited information on safe and effective neonatal rodent analgesia. The aim of this study was to evaluate the efficacy and duration of analgesia provided by buprenorphine (Bup) and lidocaine (Lid) in an incisional pain model. Male and female postnatal day three Sprague Dawley rat pups (<em>n</em>=40) were randomly assigned to one of five treatment groups: 1) Saline - 0.1 ml subcutaneous (SC)/0.01 ml saline incisional infiltration; 2) BupL - 0.025 mg/kg Bup, SC/0.01 ml saline infiltration; 3) BupH - 0.05 mg/kg Bup, SC/0.01 ml saline infiltration; 4) LidL - 2 mg/kg lidocaine infiltration/0.1 ml saline SC; and 5) LidH - LDC 4 mg/kg lidocaine infiltration/0.1 ml saline SC. Rat pups were anesthetized with sevoflurane by mask, and a 1-cm full thickness skin incision was made over the left lateral thigh. Infiltration of lidocaine or saline occurred prior to wound closure with surgical glue. Baseline thermal latency was measured 24 hr prior to surgery, and subsequently 1, 2, 4, 8, 24, and 48-hrs post-operatively using an infrared diode laser. Thermal latency in the Saline group was significantly reduced compared to baseline up to the 4 hr timepoint. Both BupL and BupH attenuated thermal hypersensitivity for at least 4 hours in this model. LidL attenuated thermal hypersensitivity for 1 hr and LidH failed to attenuate thermal hypersensitivity. No abnormal clinical signs were noted for all treatment groups throughout the study. In summary, a single pre-operative dose of buprenorphine 0.025 to 0.05 mg/kg (SC) effectively attenuated postoperative thermal hypersensitivity in 3-day old neonatal rat incisional pain model for 4 hours.</p> Erin M Katz, Monika K Huss, Katechan Jampachaisri, Cholawat Pacharinsak ##submission.copyrightStatement## Tue, 09 Mar 2021 14:27:41 +0200 Blood lactate and glucose concentrations in the femoral artery and three different veins during anaesthesia of healthy laboratory pigs <p>Blood lactate is a parameter used for monitoring pigs during prolonged anaesthesia. Here we compared blood lactate and glucose concentrations in nine anaesthetised laboratory pigs. Seventeen of the samples originated from a liver project and 15 from a kidney project. Mean and standard deviations of arterial blood lactate and glucose were compared with values for portal, renal and femoral veins by using paired Student’s t-test, paired Wilcoxon test as well as Spearman rank-order correlation. The study showed that lactate concentration was constant whether measured in blood from the femoral artery, portal vein, renal vein or femoral vein. The study showed that the origin of the blood sampled is not important and that changes in blood lactate concentration are likely to be the same throughout the cardiovascular system in healthy pigs.</p> Lisa Ann Hald, Christian Sonne, Aage Kristian Olsen Alstrup ##submission.copyrightStatement## Tue, 09 Mar 2021 00:00:00 +0200 Are female mice dehydrated during peak lactation? Effect of water and gel supplement on hydration parameters and water consumption in two strains of mice <p>Mice (<em>Mus musculus</em>) have a high basal rate of metabolism which increases during pregnancy and lactation. During peak lactation, water intake amounts to up to 65 % of the bodyweight per day. Providing water in a bottle may pose a restriction of water intake and lead to dehydration during periods of high demand, such as peak lactation. To establish if female mice are able to sustain a physiological hydration status during peak lactation, a completely randomized factorial design study was conducted with 12 RjOrl:SWISS (SWISS) and 12 C57BL/6JRj (B6) six-week old female mice in breeding. Female mice were randomly assigned to one of three groups with different watering alternatives: water bottle (Standard, n=6); water bottle + sachet with 98 % water gel (Gel, n=6); or water bottle + water bowl (Bowl, n=6). Non-mated females, provided with water bottles, served as controls (n=6). Hydration parameters [total protein (TP), hemoglobin, hematocrit, serum osmolality (Osmol), blood urea nitrogen (BUN)] and magnesium were measured in blood before mating (Pre) and during peak lactation (Peak), and at the same time points in controls. Water bottles were weighed during lactation and body weights of females and litters recorded at weaning. Data were analyzed by parametric or non-parametric methods to evaluate effects of strain, group and time point. The hydration parameters and magnesium were mostly within normal ranges in all animals at Pre and Peak. TP was lower at Peak in all lactating groups compared to Controls and to Pre (p&lt;0.01). Mice in group Bowl consumed 54 % less bottle water compared with Gel and Standard (p&lt;0.001), had 34 % lower levels of BUN than Standard and Control (p&lt;0.01) and 5 % lower serum osmolality at Peak than Pre (p&lt;0.01). <br><strong>Conclusion:</strong> Female mice are not dehydrated at peak lactation. However, they prefer to drink, and seemingly drink more water, from a bowl than from a bottle.</p> Charlotta Grims, Christina Jacobson, Patricia Hedenqvist ##submission.copyrightStatement## Mon, 17 May 2021 00:00:00 +0300