Scandinavian Journal of Laboratory Animal Sciences 2021-04-16T11:52:27+03:00 Hanna-Marja Voipio Open Journal Systems <p>Published by the Scandinavian Society for Laboratory Animal Science, Sweden</p> <p>Online ISSN <strong>2002-0112</strong></p> Buprenorphine, but not lidocaine, effectively attenuates post-operative thermal hypersensitivity in an incisional model in neonatal rats (Rattus norvegicus) 2021-03-10T11:26:09+02:00 Erin M Katz Monika K Huss Katechan Jampachaisri Cholawat Pacharinsak <p>There is limited information on safe and effective neonatal rodent analgesia. The aim of this study was to evaluate the efficacy and duration of analgesia provided by buprenorphine (Bup) and lidocaine (Lid) in an incisional pain model. Male and female postnatal day three Sprague Dawley rat pups (<em>n</em>=40) were randomly assigned to one of five treatment groups: 1) Saline - 0.1 ml subcutaneous (SC)/0.01 ml saline incisional infiltration; 2) BupL - 0.025 mg/kg Bup, SC/0.01 ml saline infiltration; 3) BupH - 0.05 mg/kg Bup, SC/0.01 ml saline infiltration; 4) LidL - 2 mg/kg lidocaine infiltration/0.1 ml saline SC; and 5) LidH - LDC 4 mg/kg lidocaine infiltration/0.1 ml saline SC. Rat pups were anesthetized with sevoflurane by mask, and a 1-cm full thickness skin incision was made over the left lateral thigh. Infiltration of lidocaine or saline occurred prior to wound closure with surgical glue. Baseline thermal latency was measured 24 hr prior to surgery, and subsequently 1, 2, 4, 8, 24, and 48-hrs post-operatively using an infrared diode laser. Thermal latency in the Saline group was significantly reduced compared to baseline up to the 4 hr timepoint. Both BupL and BupH attenuated thermal hypersensitivity for at least 4 hours in this model. LidL attenuated thermal hypersensitivity for 1 hr and LidH failed to attenuate thermal hypersensitivity. No abnormal clinical signs were noted for all treatment groups throughout the study. In summary, a single pre-operative dose of buprenorphine 0.025 to 0.05 mg/kg (SC) effectively attenuated postoperative thermal hypersensitivity in 3-day old neonatal rat incisional pain model for 4 hours.</p> 2021-03-09T14:27:41+02:00 ##submission.copyrightStatement## Blood lactate and glucose concentrations in the femoral artery and three different veins during anaesthesia of healthy laboratory pigs 2021-04-16T11:52:27+03:00 Lisa Ann Hald Christian Sonne Aage Kristian Olsen Alstrup <p>Blood lactate is a parameter used for monitoring pigs during prolonged anaesthesia. Here we compared blood lactate and glucose concentrations in nine anaesthetised laboratory pigs. Seventeen of the samples originated from a liver project and 15 from a kidney project. Mean and standard deviations of arterial blood lactate and glucose were compared with values for portal, renal and femoral veins by using paired Student’s t-test, paired Wilcoxon test as well as Spearman rank-order correlation. The study showed that lactate concentration was constant whether measured in blood from the femoral artery, portal vein, renal vein or femoral vein. The study showed that the origin of the blood sampled is not important and that changes in blood lactate concentration are likely to be the same throughout the cardiovascular system in healthy pigs.</p> 2021-03-09T00:00:00+02:00 ##submission.copyrightStatement##