Elimination of P. Aeruginosa in Mice by Treatment with Chlorine, and the use of Microbiological and PCR Analyses

  • E Mahabir Department of Comparative Medicine, Helmholtz Center, Munich, German Research Center for Environmental Health and Comparative Medicine, Center for Molecular Medicine, University of Cologne
  • D Bulian Department of Comparative Medicine, Helmholtz Center, Munich, German Research Center for Environmental Health
  • S Bensch Department of Comparative Medicine, Helmholtz Center, Munich, German Research Center for Environmental Health
  • J Schmidt Department of Comparative Medicine, Helmholtz Center, Munich, German Research Center for Environmental Health

Abstract

Unwanted micro-organisms in mice can jeopardize experimental protocols and should be eliminated if a  colony becomes infected. The opportunistic bacterium Pseudomonas aeruginosa has been shown to cause  infection in mice. In order to eliminate this bacterium from a specific-pathogen-free full barrier mouse area  at our animal facility we treated mice with 7 ppm chlorine for 7 weeks and then with 10 ppm chlorine in the  drinking water. The P. aeruginosa status of mice was examined by agar culture and PCR. The results show  that 6 months after commencement of the treatment the colony was free of P. aeruginosa

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