The Effect of Spironolactone on the Pathogenesis of Ligatureinduced Alveolar Bone Loss in Wistar Rats
Tumor necrosis factor (TNF) is a pro-inflammatory cytokine that has a straight relationship with tissue destruction in the pathogenesis of periodontitis. Inhibitory effects of TNF production have been attributed to spironolactone. The aim of this study was to evaluate the effect of spironolactone on the pathogenesis of ligature-induced alveolar bone loss in rats. Experimental periodontitis was induced in 38 Wistar rats by ligature placement in the left second maxillary molar. The contra-lateral maxillary molar served as intragroup control. Animals were randomly divided into 4 groups and treated with spironolactone (50, 100, 200 mg·kg-1) or saline. Morphometrical registration of maxillary alveolar bone was performed after 28 days of experimental periodontitis. Intra-group comparisons showed significantly higher alveolar bone loss mean values in maxillary sides with ligature (paired sample t test, p<0.05). Mean alveolar bone loss was not significantly different between groups, independently of the dosage (range: 0.63 – 0.66 mm, one-way ANOVA, p>0.05). Although spironolactone has recognized TNF-inhibitory properties, the possibility of its use on modulation of host immune-inflammatory response in periodontal disease was not confirmed.