#4 Carbonated Drinks Impact Follicle Development, Expression of Ovarian FSHR and Serum Caspase-3 in Mice
Objectives: The present study aimed to assess the effects of Coca-Cola and Pepsi-Cola on the development of ovaries and follicles, and on the reproduction of animals.
Methods: 150 female non-cycling mice weighing 20.362.28g were divided into five groups (n=30). COC-1 and COC-2 mice drank freely 50% and 100% Coca-Cola for up to 25 days. PEP-1 and PEP-2 mice received orally 50% and 100% Pepsi-Cola. Mice in the control group (CG) drank tap water. Another five mice were used as a normal control per group on day 0. Ovarian and follicle indices were measured under the microscope. Western blotting was utilized to ascertain the expression levels of FSHR protein in ovaries. Serum concentrations of caspase-3, epidermal growth factor (EGF) and vascular endothelial growth factor receptor (VEGFR) were measured by ELISA.
Results: The ovarian weight of PEP-2 was significantly lower than that of CG (P<0.05) on day 25. Ovarian cortex thickness (OCT) of PEP-2 was significantly reduced in comparison with CG (P<0.01). In the COC-2 group, values of follicle longitudinal diameter (FLD), follicle transverse diameter (FTD), follicle wall thickness (FWT), oocyte longitudinal diameter (OLD) and oocyte transverse diameter (OTD) were less than those of CG on day 25. FWT and OLD of PEP-1 were reduced as compared to CG. In COC and PEP groups, numbers of POF and MF were reduced slightly. Follicles developed poorly. FSHR protein was decreased slightly and EGF levels increased in Coca-Cola treated or Pepsi-Cola treated mice. Serum caspase-3 levels of COC-1 and PEP-1 were significantly lower than those of CG.
Conclusion: Coca-Cola and Pepsi-Cola treatment could reduce ovarian weights, inhibit the ovarian cortex thickness and impair the development of follicles.