#3 Refinement of a hematogenous localized osteomyelitis model in pigs

  • A K O Alstrup Department of Nuclear Medicine & PET-Centre, Aarhus University Hospital, Aarhus
  • K M Nielsen Department of Veterinary Disease Biology, University of Copenhagen, Copenhagen and Department of Nuclear Medicine, Aalborg University Hospital, Aalborg
  • H C Sch Department of Clinical Microbiology, Aalborg University Hospital, Aalborg and Department of Clinical Medicine, Aalborg University, Aalborg
  • S B Jensen Department of Nuclear Medicine, Aalborg University Hospital, Aalborg and Department of Chemistry and Biochemistry, Aalborg University, Aalborg
  • P Afzelius Department of Diagnostic Imaging, Copenhagen University Hospital, North Zealand, Hillerød
  • P S Leifsson Department of Veterinary Disease Biology, University of Copenhagen, Copenhagen
  • O L Nielsen Department of Veterinary Disease Biology, University of Copenhagen, Copenhagen

Abstract

We have previously developed a model of localized osteomyelitis by injecting Staphylococcus aureus (S. aureus) unilaterally into the femoral artery of juvenile domestic pigs (Johansen et al., 2012; Nielsen et al., 2015). We used this model for the evaluation of bone-infection tracers applicable for positron emission tomography (PET) and single-photon emission computed tomography (SPECT) (Nielsen et al., 2015). However, several of the 40 kg pigs were euthanized prior to PET and SPECT scanning due to lameness, shallow respiration, fever and anorexia; the last three clinical signs indicating dissemination of S. aureus to the lungs and other internal organs. We therefore decided to refine our model in order to improve the success rate. We speculated that younger pigs might respond differently to inoculations. A total of ten female domestic pigs were included in our study, three with a body weight of 40 kg (

Published
2016-04-04
Section
Articles