#6 Stability of Clinical Chemistry and Hematological Analytes in Preserved Plasma and Blood Obtained from Wistar Rats

M Singh, R Pandya, S Chandra, S K Sharma


Blood samples obtained from experimental animals often need preservation due to several technical constraints. The present study was aimed to determine the effect of storage temperature and time on the stability of analytes in whole blood and plasma samples obtained from Wistar rats. Aspartate amino transferase, alkaline phosphatase, cholesterol, triglycerides, creatinine, urea, glucose, total protein, total bilirubin, phosphorous, sodium, potassium and chloride did not show statistically significant changes in plasma preserved at –20 °C up to 4 weeks. However, alanine aminotransferase decreased by 4th week and gamma glutamyl transferase showed variance at different time points, while a statistically significant decrease was noted in albumin and calcium levels from the first week. A marked increase in LDH activity was noted after storage for 2 and 4 weeks. No substantial changes in complete blood count were noted. However, an increase was recorded in mean corpuscular volume at 72 hr and mean corpuscular hemoglobin at 48 hr and 72 hr in blood stored at 4 ±1°C. To conclude, the preservation temperatures of –20 ± 2 °C for plasma up to 4 weeks and 4 ±1°C up to 96 hr for whole blood in Wistar rats did not remarkably affect the stability of analytes except for LDH and may be considered for laboratory investigations if warranted.

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