#3 Correlation between synthesis of α2-macroglobulin in hepatocytes and changes in serum cytokine levels in rats after infl ammatory stimulation
The time course of α2-macroglobulin (α2M) synthesis in rat liver was investigated using immunohistochemistry. Furthermore, correlations between synthesis of α2M in hepatocytes and interleukin (IL)-6 and cytokine-induced neutrophil chemoattractant-1 (CINC-1), which are considered to contribute to the production of α2M, were evaluated. The presence of α2M in the liver was investigated by immunohistochemistry, and serum levels of α2M, IL-6 and CINC-1 were measured by enzyme-linked immunosorbent assay. α2M was not detected in the liver before injection of turpentine oil. α2M was detected throughout the liver at 12 hours after injection of turpentine oil, when high serum levels of IL-6 and CINC-1 were observed. α2M was distributed mainly around the central vein of the liver at 36 hours. Only small amounts of α2M were detected in the liver at 48 hours, when peak serum levels of α2M were observed. Synthesis of α2M in hepatocytes peaked long before peak α2M serum levels were seen. In conclusion, α2M was considered to be synthesized in response to stimulation by IL-6 and CINC-1.
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