Proliferative Response of Hamster CD4+ T Cells after Different Mitogenic Stimulation

  • Salvador Fonseca-Coronado Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México
  • Eugenio Del Valle-Espinosa Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México
  • Sigifredo Pedraza-Sánchez Unidad de Bioquímica, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Secretaria de Salud
  • Ana Flisser Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México

Abstract

The measurement of effector cells involved in immune responses is an important parameter in various  fields of lymphocyte research using the Syrian Golden hamster as the experimental model. The aim of  the present study was to identify the percentage of peripheral hamster CD4+ cells that undergo proliferation  and the number of cell divisions induced by four different mitogenic compounds: phorbol-myristate  acetate plus ionomycin (PMA/ionomycin), concanavalin A (Con A), phytohaemagglutinin (PHA) and the  superantigen Staphylococcal enterotoxin B (SEB). The proliferative response was investigated by the use of  the cross-reactive mouse anti-CD4 antibody (clone H129.9) and analyzed by flow cytometry using the dye  carboxyfluorescein diacetate succinimydil ester (CFSE). PMA/ionomycin induced the highest proliferative  response of CD4+ cells. Interestingly, the down-regulation in the CD4 expression described after PMA/  ionomycin activation both in human and in mouse lymphocytes was not seen in hamster cells. Stimulation  with SEB and with Con A also induced up to 6 to 7 proliferation cycles in cells, whereas PHA induced the  lowest proliferation. These results provide useful tools for the study of CD4+ cells in the hamster model. 

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