Technical Report Scintigraphic Evaluation of Bone Formation in Göttingen Minipigs

  • M L Schwarz Orthopaedic and Trauma Centre, Laboratory for Biomechanics and Experimental Orthopaedics, University Medical Centre Mannheim, University of Heidelberg
  • R Seidling Orthopaedic and Trauma Centre, Laboratory for Biomechanics and Experimental Orthopaedics, University Medical Centre Mannheim, University of Heidelberg
  • E Mauermann Orthopaedic and Trauma Centre, Laboratory for Biomechanics and Experimental Orthopaedics, University Medical Centre Mannheim, University of Heidelberg
  • A Werner Institute of Clinical Radiology and Nuclear Medicine, University Medical Centre Mannheim, University of Heidelberg
  • V Steil Department of Radiation Oncology, University Medical Centre Mannheim, University of Heidelberg
  • E Forsch Department of Anesthesiology and Intensive Care Medicine, Medical Faculty Mannheim, University of Heidelberg
  • U Obertacke Orthopaedic and Trauma Centre, Laboratory for Biomechanics and Experimental Orthopaedics, University Medical Centre Mannheim, University of Heidelberg
  • K Becker IBF, University of Heidelberg
  • L Lehmann Orthopaedic and Trauma Centre, Laboratory for Biomechanics and Experimental Orthopaedics, University Medical Centre Mannheim, University of Heidelberg

Abstract

In experiments and processes requiring the application of nuclear tracers in large animals, statutory provisions  and safety standards as well as a variety of techniques have to be regarded and employed. 

In order to sufficiently analyze questions pertaining to osseointegration as well as the possibility of ectopic  bone formation in Göttingen minipigs, we decided to use scintigraphic examinations using 99mTc-HDP  (Technetium - hydroxymethane diphosphonate). In this study, metallic implants coated in different forms  with rhBMP-2 (recombinant human bone morphogenetic protein-2) were surgically introduced into the  pigs’ femora. A total of 26 adult female minipigs (Ellegard, Dalmose, Denmark) averaging 40 months in  age were post-surgically evaluated through the application of a radionuclide and its subsequent distribution  using a scintillation camera. Each animal received approximately 10 MBq/kg BW (mega Becquerel per  kilogram bodyweight). 

This paper describes the procedures of anaesthesia, the quite challenging transvaginal- urethral catheterization,  the application of a catheter in the jugular vein, the radionuclide injection and the disposal of the  sacrificed animals under statutory provisions and safety standards. 

The technical report reveals that the scintigraphic evaluation in large animal experiments is a practicable  – yet sophisticated – method of examination and also strives to encourage further research groups to implement  this elegant procedure. 

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